Abstract：The current method for anti-doping testing in sports relies on urine analysis and venous blood samples provided by athletes. Recently, the World Anti-Doping Agency has implemented a new testing strategy: dried blood spot (DBS). This method was trialed at the Tokyo 2020 Olympic Games then officially utilized for routine testing at the 2022 Beijing Winter Olympic Games. This paper aims to explore and discuss the issue from the perspective of doping control by examining official guidelines and reports and reviewing the latest scientific research literature. The results show that DBS collection is relatively straightforward, fast, produces very little pain, and does not require medical personnel. Use of DBS testing is expected to increase. A variety of sampling devices are being developed to absorb minute amounts of blood homogeneously and let them dry, yet there is still no preference for a specific manufacturer. In terms of analysis, DBS can currently be used only for the detection of non-threshold substances without minimum reporting levels. That is, the substances prohibited in-competition and the drugs misused for therapeutic purposes are excluded. One of the greatest advantages of the DBS testing is that it can be used to detect 'anabolic steroid preparations (esters)'. These compounds are highly stable only in a dry matrix, hence the detection of their unchanged state in DBS samples can be considered as direct evidence for doping. This paper concludes that DBS can fill the unmet needs of the current testing strategy particularly in today's increasingly diverse situation of drug misuse. More research on this new technology is warranted to develop multiple applications such as the Athlete Biological Passport. DBS testing is anticipated to be implemented progressively at all levels of sports games in the future to protect fair competition. (Full text)